Assessment of Pseudoaffinity Chromatography Using Textile Dyes for Isolation of Buffalo Pituitary Luteinizing Hormone

Table 2

Fold purification achieved of the various eluted fractions obtained when crude LH was loaded on different agarose-linked textile dyes, and the nature of the eluants was changed.

S. no.	Name of the fraction	Immunoreactive LH content (in ng)	Fold purification
(1)	Buffalo LH (buLH)	6	—
(2)	Cibacron ethylene glycol fraction (CB2)	200	3
(3)	Cibacron NaCl fraction (CB3)	35	17.14
(4)	Reactive blue ethylene glycol fraction (BL2)	250	2.4
(5)	Reactive blue NaCl fraction (BL3)	60	10
(6)	Reactive brown ethylene glycol fraction (BR2)	200	3
(7)	Reactive brown NaCl fraction (BR3)	400	1.5
(8)	Reactive yellow unbound fraction (Y1)	25	24
(9)	Reactive yellow ethylene glycol fraction (Y2)	400	1.5
(10)	Reactive yellow NaCl fraction (Y3)	12	50
(11)	Reactive green ethylene glycol fraction (GR2)	110	5.4
(12)	Reactive green NaCl fraction (GR3)	1000	0.6