Cytotoxicity effect of AT-01 peptide and cisplatin at 1 μM on U87 human glioblastoma, A431 skin cancer, and HepG2 liver cancer cell lines. Cancer cells (1 × 104) were seeded, and after 24 hours the cells were treated separately with 1 μM concentration of AT-01 peptide and cisplatin as a positive control. The viability of the cells was estimated by using MTT assay on the basis of formazan formed, which was detected spectrophotometrically by measuring optical density at 595 nm at 24 hours, and % cell cytotoxicity was determined [28].
