International Scholarly Research Notices / 2012 / Article / Tab 1

Research Article

Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications

Table 1

Comparison of detection limits of 3 serum proteins with the current HPLC-LIF system and available data from literature.

Sl. No.ProteinsLiterature Limit of DetectionCurrent HPLC-LIF Limit of Detection

1Creatine Phosphokinase (CPK)8 U/L337.3 (0.05) fmoles
2Ferritin0.25 ng/mL30 (52.1 picomoles)76 (0.1) fmoles
3Human Serum Albumin (HSA)17.32 ng/mL34 (0.26 nmoles)11.6 (0.01) fmoles
4Transferrin50 pg/mL31 (0.625 nmoles)2.1 (0.001) fmoles
5Insulin2 ppm28, 30 ppm2917.4 (0.01) fmoles

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