Table of Contents
ISRN Toxicology
Volume 2012, Article ID 693489, 5 pages
Research Article

Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method

1Toxicology, Aptus Biosciences Private Limited, SVS Medical College Campus, Yenugonda, Andhra Pradesh, Mahabubnagar 509 002, India
2Quality Assurance Unit, Aptus Biosciences Private Limited, SVS Medical College Campus, Yenugonda, Mahabubnagar 509 002, India

Received 26 December 2011; Accepted 15 January 2012

Academic Editor: P. Urban

Copyright © 2012 Gajanan Rajpal Deshmukh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present paper is an attempt to investigate the eye irritation potential of aqueous leaf extract of Achyranthes aspera by in vitro, Hen's Egg Chorioallantoic Membrane Test (HET-CAM) and in vivo acute eye irritation test in rabbits. The irritation score (IS) obtained after treatment of the extract on HET-CAM is 0.07 and that of in rabbits is 0.55, Which does not comes under either category 1 or 2 as per the harmonized integrated classification system. The aqueous extract of Achyranthes aspera showed no eye irritation properties both in vitro and in vivo methods when compared with negative control whereas positive controls showed eye irritation potential.