C3b-Independent Complement Activation in Ischemia/Reperfusion Mesenteric Tissue Injury in Autoimmune Prone (B6.MRL/lpr) Mice
Figure 2
Analysis of C3 depletion in IR model of inflammatory tissue injury. Injury scores generated from H&E stained transverse sections of intestinal tissue are analyzed. (a) Injury scores for treatment groups in each strain are graphed. Five-month old B6.MRL/lpr mice displayed significant intestinal IRI damage despite depletion of complement with CVF. The degree of injury was comparable to the injury that occurs in these mice without any complement inhibition. In contrast, depletion of C3 in CVF treated C57BL/6 mice effectively abrogated tissue damage in the IRI model. By one-way ANOVA testing, specifically the Kruskal-Wallis test indicated significance between treatment groups in both the B6.MRL/lpr set () and the C57Bl/6 set (). Data represents analysis of 5 animals per group. (b) H&E images illustrate the representative injury level detected in the CVF depleted IR groups of C57Bl/6 and B6.MRL/lpr strains.
