C3b-Independent Complement Activation in Ischemia/Reperfusion Mesenteric Tissue Injury in Autoimmune Prone (B6.MRL/lpr) Mice
Figure 4
Detection of C5a and thrombin in C3 deleted B6.MRL/lpr animals. Images of immunofluorescence staining of transverse intestinal tissue sections are presented. (a) C5a is not detected in panels 1: sham tissue, or panel 3: C1 esterase treated IR tissue. C5a is detected in panel 2: IR tissue and panel 4: CVF treated IR tissue. (b) Thrombin staining is detected in the IR tissue (left panel) and CVF tissue (center panel), but is absent in the C1 esterase-IR tissue (right panel). The images used are representative images taken from analyses of tissue sections from each animal in the group, with a group size of 5 animals.
