Table of Contents
ISRN Pharmacology
Volume 2012, Article ID 709860, 6 pages
http://dx.doi.org/10.5402/2012/709860
Research Article

The New Method Developed for Evaluation of Anthelmintic Activity by Housefly Worms and Compared with Conventional Earthworm Method

1Department of Pharmaceutical Biotechnology, Abhilashi College of Pharmacy, Tanda, Ner-Chowk, Mandi-175008, Himachal Pradesh, India
2School of Pharmacy and Engineering & Technology, Chail-Chowk, Mandi, Himachal Pradesh, India
3Department of Pharmaceutical Biotechnology, Andhra University College of Pharmacy, Andhra University, Vishakapattanam, Andhra Pradesh, India

Received 27 November 2011; Accepted 11 January 2012

Academic Editors: M. Alkondon and D. K. Miller

Copyright © 2012 V. Murugamani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Evaluation of anthelmintic activity of any drug when carried out in laboratory conditions by using the isolated worms from nature cannot be adaptable with artificial laboratory conditions. Therefore, the present study aims at developing a new adaptable method for evaluation of anthelmintic activity. The present anthelmintic activity study reveals a new methodology with housefly worms cultured in laboratory conditions that resemble parasitic pinworms found in human being. We studied the anthelmintic activities of various drugs on housefly worms and earthworms. The results showed that the housefly worms had taken more time for paralysis and death. Even after paralysis the time taken for death is more in housefly worms in spite of smaller size and lesser weight of the worms compared to earthworms. The study concluded that the earthworms have not adapted to the artificial laboratory conditions leading to erratic results. Therefore, culturing of housefly worms was carried out to evaluate the anthelmintic activity and found an easy, prominent, eco-friendly, and reproducible method in all aspects such as equal age, size, and weight of worms used for the experiment.