Doxorubicin and NRG-1/erbB4-Deficiency Affect Gene Expression Profile: Involving Protein Homeostasis in Mouse

Figure 5

Protein ubiquination in cardiomyocytes. Transverse ventricular sections of nontreated WT and KO and doxorubicin-treated WT and KO hearts (WTD, KOD) () were immunnostained with antibodies against ubiquitin, revealed with biotin-coupled antiolyclonal rabbit and FITC-coupled streptavidin (green). The membrane was stained with wheat germ agglutinin (TRICT-coupled WGA, red) and nuclei with DAPI (blue). The images were scanned with fluorescent microscope (upper panel), original magnification 400x. Scale bar: 100 m. Note ubiquitin-protein aggregates (arrows) and ubiquitin-labeled proteins adjacent to the membrane in erbB4-KOD. Detailed images of KOD sections with large ubiquitin-posistive protein aggregates were obtained by confocal microscopy (lower panel).