Table of Contents
ISRN Vascular Medicine
Volume 2012 (2012), Article ID 769092, 10 pages
http://dx.doi.org/10.5402/2012/769092
Research Article

Adiponectin Fails in Improving Angiogenic Repair in Streptozocin-Treated or L e p r d b / d b Mice after Hind Limb Ischemia

1Center for Geriatric Medicine, Bezirksklinikum Regensburg, Universitätsstraße 84, 93053 Regensburg, Germany
2Department of Internal Medicine III, Heidelberg University, 69117 Heidelberg, Germany
3Translational & Experimental Medicine, Luxembourg Centre for Systems Biomedicine, Luxembourg
4Department of Medicine II, Saarland University, Medical Center, 66421 Homburg, Germany

Received 10 April 2012; Accepted 12 June 2012

Academic Editors: A. Habib, A. Paolicchi, and A. Shaish

Copyright © 2012 Kurt Belisle et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. Type 1 and 2 diabetes carry risk factors for the development of microvascular diseases with associated impairment of angiogenic repair. Here, we investigated whether adiponectin, an adipocyte-specific adipocytokine with antiatherosclerotic and antidiabetic properties, regulates angiogenic repair in response to tissue ischemia in L e p r d b / d b and streptozocin-treated diabetic mouse models. Methods. Adenoviral vectors containing the gene for β-galactosidase, full-length mouse adiponectin, and dominant-negative AMPKα2 were used in streptozocin-treated male L e p r d b / d b mice, after which hind limb blood flow was measured using a laser doppler blood flow analyzer. Results. The angiogenic repair of ischemic hind limbs was impaired in both streptozocin-treated and L e p r d b / d b mice compared to wild-type mice as evaluated by laser doppler flow and capillary density analyses. Adenovirus-mediated administration of adiponectin accelerated angiogenic repair after hind limb ischemia in WT mice, but not in L e p r d b / d b mice or mice treated with streptozocin. In vitro experiments using HUVECs highlighted the antiapoptotic and proangiogenic properties of adiponectin but could not demonstrate accelerated differentiation of endothelial cells into tube-like structures at elevated glucose levels. Conclusions. External administration of adiponectin at elevated glucose levels may not be useful in the treatment of diabetes mellitus-related vascular deficiency diseases.