A Novel Membrane Protein-Specific Serine/Threonine Kinase: Tissue Distribution and Role in Sperm Maturation

Figure 1

Immunoassay of CIK in maturing caprine sperm (i.e., caput, corpus, and cauda sperm) cytosol and plasma membrane: sperm cytosol and plasma membrane fractions were prepared as described in Section 2. Sperm plasma membranes were isolated by two-phase polymer method. Distribution of CIK in the sperm fractions was determined by ELISA under standard assay condition. The data shown are Mean ± s.e.m. of 5 experiments. “C” denotes cytosol and “M” denotes membrane.