Figure 7: Blockade of IFNγ response genes in RMS cannot be abrogate by demethylation. Survival of RMS cell lines after 5′Aza (dark grey bars) and combined treatment of 5′Aza and IFNγ (light grey bars); cells were incubated for 72 h with 100 μM 5′aza 2′deoxycytidine (5′Aza) in starvation media with 1% FCS before treatment with IFNγ as described; survival of nontreated tumour cells (0 h) was set 100%. *P < 0.05; **P < 0.01; ***P < 0.001.