Research Article

Signal Peptidase Complex Subunit 1 and Hydroxyacyl-CoA Dehydrogenase Beta Subunit Are Suitable Reference Genes in Human Lungs

Table 2

Stability of housekeeping genes generated by NormFinder [11]. By extracting the pool of Z-transformed probes intensities with the lowest 10% CV, the remaining 5,458 probes (out of 54,676 probes) were further selected to eliminate undefined genes. The selected 743 genes was input in NormFinder [11] to generate the stability value for each gene as a direct measure for the estimated expression variation and rank them accordingly. SPCS1 and HADHB, which were found to have the lowest CV values, demonstrated the lowest stability index and ranked as first and second, respectively.

Gene symbolsNormFinder stability indexRank

SPCS10.3261
HADHB0.3552
EIF2A0.691371
PPIB0.862630
PPIB0.867634
RPL13A0.651305
RPL13A0.811548
RPL13A0.832587
RPL13A0.911689
RPL13A0.950730
RPL320.739454
RPL40.723428
RPL40.789518
RPL40.805541
RPS4X0.777499
RPS4X0.838592