Table of Contents
ISRN Analytical Chemistry
Volume 2012, Article ID 809256, 5 pages
Research Article

Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence

1Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, China
2Shaanxi General Team of China Construction Materials and Geological Prospecting Center, Xi'an 710003, China

Received 11 March 2012; Accepted 6 May 2012

Academic Editors: M. E. Diaz-Garcia, P. Kingshott, M. Özgür, and M. L. Villarreal

Copyright © 2012 Jiajia Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A sensitive chemiluminescence (CL) method for the determination of hesperetin at nanogram levels was first presented. It was based on the inhibitory effect of hesperetin on luminol-dissolved oxygen CL reaction in a flow injection system. The decrements of CL intensity were logarithm over the concentrations of hesperetin in a range of 0.5 to 1000 ng mL−1, with a detection limit of 0.2 ng mL−1 ( 3 𝜎 ) and the relative standard deviations lower than 2.1%. At a flow rate of 2.0 mL min−1, the whole determining performance including sampling and washing could be accomplished in 30 s, giving a sampling efficiency of 120 h−1. The proposed method was applied successfully to the direct hesperetin determination in human serum with recoveries from 97.0 to 106.6%, and indirect hesperidin quantification in 2.5 g pericarpium citri reticulatae showing that the content is 8 . 1 ± 0 . 2 %. The possible CL mechanism of luminol-dissolved oxygen-hesperetin reaction was also discussed in detail.