Figure 3: NCI8642 is able to revert DKK1 inhibition of the Wnt pathway. (a) Luciferase activity detected in PC12-TCF-Luc cells stimulated with Wnt3a alone and in presence of increasing volumes of DKK1 CM. (b) Effect of NCI8642 on Wnt3a and DKK1 treated cells. Open square: basal signal; open triangle: cells treated with Wnt3a CM; open circle: cells treated with Wnt3a, DKK1 CM, and DMSO 1%; close symbols: cells treated with serial dilutions of NCI8642 (up to 50 μM), either in the presence (close circles) or in the absence (close triangles) of DKK1 CM.