Collagen Extraction from Malaysian Cultured Catfish (Hybrid Clarias Sp.): Kinetics and Optimization of Extraction Conditions Using Response Surface Methodology

Table 1

Response surface CCD and results for yield of PSC extraction.

No.	𝑋1,; acetic acid concentration (M)	𝑋2,; liquid to solid ratio; (mL/g)	𝑋3,; stirring speed; (rpm)	Y,; yield of extraction PSC (mg/g)
1	−1 (0.1)	−1 (20)	−1 (300)	51.04 ± 0.85
2	1 (0.9)	−1 (20)	−1 (300)	116.54 ± 3.35
3	−1 (0.1)	1 (30)	−1 (300)	50.93 ± 0.35
4	1 (0.9)	1 (30)	−1 (300)	151.67 ± 5.37
5	−1 (0.1)	−1 (20)	1 (500)	100.51 ± 2.17
6	1 (0.9)	−1 (20)	1 (500)	183.45 ± 3.34
7	−1 (0.1)	1 (30)	1 (500)	60.20 ± 1.51
8	1 (0.9)	1 (30)	1 (500)	160.82 ± 4.87
9	−1 (0.1)	0 (25)	0 (400)	110.50 ±4.55
10	1 (0.9)	0 (25)	0 (400)	201.82 ± 6.78
11	0 (0.5)	−1 (20)	0 (400)	190.91 ± 3.38
12	0 (0.5)	1 (30)	0 (400)	170.22 ±1.66
13	0 (0.5)	0 (25)	−1 (300)	139.55 ± 0.54
14	0 (0.5)	0 (25)	1 (500)	220.77 ± 9.88
15	0 (0.5)	0 (25)	0 (400)	217.56 ± 7.56
16	0 (0.5)	0 (25)	0 (400)	203.67 ± 5.73
17	0 (0.5)	0 (25)	0 (400)	212.45 ± 10.51
18	0 (0.5)	0 (25)	0 (400)	206.45 ± 5.55
19	0 (0.5)	0 (25)	0 (400)	222.76 ± 9.37
20	0 (0.5)	0 (25)	0 (400)	219.78 ± 8.46