Table of Contents
ISRN Pharmacology
Volume 2012, Article ID 843569, 8 pages
http://dx.doi.org/10.5402/2012/843569
Research Article

Vitamins E and C Alleviate the Germ Cell Loss and Oxidative Stress in Cryptorchidism When Administered Separately but Not When Combined in Rats

1Department of Physiology, College of Health Sciences, Ladoke Akintola University of Technology, PMB 4000, Ogbomoso, Oyo, Nigeria
2Department of Physiology, Faculty of Medicine, Kogi State University, PMB 1008, Anyigba, Kogi, Nigeria

Received 23 September 2012; Accepted 10 October 2012

Academic Editors: T. Irie and D. K. Miller

Copyright © 2012 Ayobami Oladele Afolabi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. K. M. Fairfield and R. H. Fletcher, “Vitamins for chronic disease prevention in adults: scientific review,” Journal of the American Medical Association, vol. 287, no. 23, pp. 3116–3126, 2002. View at Google Scholar · View at Scopus
  2. M. C. Polidori, P. Mecocci, S. E. Browne, U. Senin, and M. F. Beal, “Oxidative damage to mitochondrial DNA in Huntington's disease parietal cortex,” Neuroscience Letters, vol. 272, no. 1, pp. 53–56, 1999. View at Publisher · View at Google Scholar · View at Scopus
  3. M. J. Stampfer, C. H. Hennekens, J. E. Manson, G. A. Colditz, B. Rosner, and W. C. Willett, “Vitamin E consumption and the risk of coronary disease in women,” New England Journal of Medicine, vol. 328, no. 20, pp. 1444–1449, 1993. View at Publisher · View at Google Scholar · View at Scopus
  4. S. H. Lee, T. Oe, and I. A. Blair, “Vitamin C-induced decomposition of lipid hydroperoxides to endogenous genotoxins,” Science, vol. 292, no. 5524, pp. 2083–2086, 2001. View at Publisher · View at Google Scholar · View at Scopus
  5. B. Halliwell, “Vitamin C: poison, prophylactic or panacea?” Trends in Biochemical Sciences, vol. 24, no. 7, pp. 255–259, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Meydani, W. J. Evans, G. Handelman et al., “Protective effect of vitamin E on exercise-induced oxidative damage in young and older adults,” American Journal of Physiology, vol. 264, no. 5, pp. R992–R998, 1993. View at Google Scholar · View at Scopus
  7. D. Zhang, S. Okada, Y. Yu, P. Zheng, R. Yamaguchi, and H. Kasai, “Vitamin E inhibits apoptosis, DNA modification, and cancer incidence induced by iron-mediated peroxidation in Wistar rat kidney,” Cancer Research, vol. 57, no. 12, pp. 2410–2414, 1997. View at Google Scholar · View at Scopus
  8. M. S. Cooke, M. D. Evans, I. D. Podmore et al., “Novel repair action of vitamin C upon in vivo oxidative DNA damage,” FEBS Letters, vol. 439, no. 3, pp. 363–367, 1998. View at Publisher · View at Google Scholar · View at Scopus
  9. K. Sato, E. Niki, and H. Shimasaki, “Free radical-mediated chain oxidation of low density lipoprotein and its synergistic inhibition by vitamin E and vitamin C,” Archives of Biochemistry and Biophysics, vol. 279, no. 2, pp. 402–405, 1990. View at Publisher · View at Google Scholar · View at Scopus
  10. A. C. Chan, “Partners in defense, vitamin E and vitamin C,” Canadian Journal of Physiology and Pharmacology, vol. 71, no. 9, pp. 725–731, 1993. View at Google Scholar · View at Scopus
  11. J. R. Britton, I. D. Pavord, K. A. Richards et al., “Dietary antioxidant vitamin intake and lung function in the general population,” American Journal of Respiratory and Critical Care Medicine, vol. 151, no. 5, pp. 1383–1387, 1995. View at Google Scholar · View at Scopus
  12. M. Meydani, W. J. Evans, G. Handelman et al., “Protective effect of vitamin E on exercise-induced oxidative damage in young and older adults,” American Journal of Physiology, vol. 264, no. 5, pp. R992–R998, 1993. View at Google Scholar · View at Scopus
  13. J. T. Salonen, K. Nyyssönen, R. Salonen et al., “Antioxidant Supplementation in Atherosclerosis Prevention (ASAP) study: a randomized trial of the effect of vitamins E and C on 3-year progression of carotid atherosclerosis,” Journal of Internal Medicine, vol. 248, no. 5, pp. 377–386, 2000. View at Publisher · View at Google Scholar · View at Scopus
  14. H. N. Lim, E. Rajpert-De Meyts, N. E. Skakkebæk, J. R. Hawkins, and I. A. Hughes, “Genetic analysis of the INSL3 gene in patients with maldescent of the testis,” European Journal of Endocrinology, vol. 144, no. 2, pp. 129–137, 2001. View at Google Scholar · View at Scopus
  15. M. Ahotupa and I. Huhtaniemi, “Impaired detoxification of reactive oxygen and consequent oxidative stress in experimentally cryptorchid rat testis,” Biology of Reproduction, vol. 46, no. 6, pp. 1114–1118, 1992. View at Publisher · View at Google Scholar · View at Scopus
  16. A. L. Villumsen and B. Zachau-Christiansen, “Spontaneous alterations in position of the testes,” Archives of Disease in Childhood, vol. 41, no. 216, pp. 198–200, 1966. View at Google Scholar · View at Scopus
  17. P. E. Ansell, V. Bennet, D. Bull et al., “Cryptorchidism: a prospective study of 7500 consecutive male births, 1984–8,” Archives of Disease in Childhood, vol. 67, no. 7, pp. 892–899, 1992. View at Google Scholar · View at Scopus
  18. K. Sijstermans, W. W. M. Hack, L. M. Van Der Voort-Doedens, R. W. Meijer, and K. Haasnoot, “Puberty stage and spontaneous descent of acquired undescended testis: implications for therapy?” International Journal of Andrology, vol. 29, no. 6, pp. 597–602, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. H. E. Virtanen and J. Toppari, “Epidemiology and pathogenesis of cryptorchidism,” Human Reproduction Update, vol. 14, no. 1, pp. 49–58, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. G. S. Berkowitz, R. H. Lapinski, S. E. Dolgin, J. G. Gazella, C. A. Bodian, and I. R. Holzman, “Prevalence and natural history of cryptorchidism,” Pediatrics, vol. 92, no. 1, pp. 44–49, 1993. View at Google Scholar · View at Scopus
  21. K. A. Boisen, M. Kaleva, K. M. Main et al., “Difference in prevalence of congenital cryptorchidism in infants between two Nordic countries,” The Lancet, vol. 363, no. 9417, pp. 1264–1269, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. B. Buemann, H. Henriksen, A. L. Villumsen, A. Westh, and B. Zachau-Christiansen, “Incidence of undescended testis in the newborn,” Acta Chirurgica Scandinavica. Supplementum, vol. 283, pp. 289–293, 1961. View at Google Scholar
  23. P. Ghirri, C. Ciulli, M. Vuerich et al., “Incidence at birth and natural history of cryptorchidism: a study of 10,730 consecutive male infants,” Journal of Endocrinological Investigation, vol. 25, no. 8, pp. 709–715, 2002. View at Google Scholar · View at Scopus
  24. V. K. Mital and B. K. Garg, “Undescended testicle,” The Indian Journal of Pediatrics, vol. 39, no. 5, pp. 171–174, 1972. View at Publisher · View at Google Scholar · View at Scopus
  25. R. T. Preikša, B. Žilaitiene, V. Matulevičius et al., “Higher than expected prevalence of congenital cryptorchidism in Lithuania: a study of 1204 boys at birth and 1 year follow-up,” Human Reproduction, vol. 20, no. 7, pp. 1928–1932, 2005. View at Publisher · View at Google Scholar · View at Scopus
  26. C. G. Scorer, “The descent of the testis,” Archives of Disease in Childhood, vol. 39, pp. 605–609, 1964. View at Google Scholar · View at Scopus
  27. M. K. Thong, C. T. Lim, and H. Fatimah, “Undescended testes: incidence in 1002 consecutive male infants and outcome at 1 year of age,” Pediatric Surgery International, vol. 13, no. 1, pp. 37–41, 1998. View at Publisher · View at Google Scholar · View at Scopus
  28. R. C. Benson, C. M. Beard, P. P. Kelalis, and L. T. Kurland, “Malignant potential of the cryptorchid testis,” Mayo Clinic Proceedings, vol. 66, no. 4, pp. 372–378, 1991. View at Google Scholar · View at Scopus
  29. C. Krausz, L. Quintana-Murci, M. Fellous, J. P. Siffroi, and K. McElreavey, “Absence of mutations involving the INSL3 gene in human idiopathic cryptorchidism,” Molecular Human Reproduction, vol. 6, no. 4, pp. 298–302, 2000. View at Google Scholar · View at Scopus
  30. S. P. Chaki, M. M. Misro, D. Ghosh, D. K. Gautam, and M. Srinivas, “Apoptosis and cell removal in the cryptorchid rat testis,” Apoptosis, vol. 10, no. 2, pp. 395–405, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. T. T. Turner, H. J. Bang, and J. J. Lysiak, “Experimental testicular torsion: reperfusion blood flow and subsequent testicular venous plasma testosterone concentrations,” Urology, vol. 65, no. 2, pp. 390–394, 2005. View at Publisher · View at Google Scholar · View at Scopus
  32. M. M. Misro, S. P. Chaki, and D. K. Gautam, “Germ cell death and their removal during initial stages of testicular ischemia and cryptorchidism: a comparative analysis,” Indian Journal of Experimental Biology, vol. 43, no. 11, pp. 1080–1087, 2005. View at Google Scholar · View at Scopus
  33. V. Peltola, I. Huhtaniemi, and M. Ahotupa, “Abdominal position of the rat testis is associated with high level of lipid peroxidation,” Biology of Reproduction, vol. 53, no. 5, pp. 1146–1150, 1995. View at Publisher · View at Google Scholar · View at Scopus
  34. Y. C. Li, X. Q. Hu, L. J. Xiao et al., “An oligonucleotide microarray study on gene expression profile in mouse testis of experimental cryptorchidism,” Frontiers in Bioscience, vol. 11, no. 2, pp. 2465–2482, 2006. View at Publisher · View at Google Scholar · View at Scopus
  35. M. Ikeda, H. Kodama, J. Fukuda et al., “Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress,” Biology of Reproduction, vol. 61, no. 2, pp. 393–399, 1999. View at Publisher · View at Google Scholar · View at Scopus
  36. J. Dupont, P. J. White, M. P. Carpenter et al., “Food uses and health effects of corn oil,” Journal of the American College of Nutrition, vol. 9, no. 5, pp. 438–470, 1990. View at Google Scholar · View at Scopus
  37. A. O. Afolabi, O. Aluko, O. Oyewopo, B. M. Olabinri, O. Olotu, and Y. Raji, “Trino IB ameliorates the oxidative stress of cryptorchidism in the rat,” African Journal of Biotechnology, vol. 8, no. 7, pp. 1183–1187, 2009. View at Google Scholar · View at Scopus
  38. A. O. Akpantah, A. A. Oremosu, M. O. Ajala, C. C. Noronha, and A. O. Okanlawon, “The effect of crude extract of Garcinia kola seed on the histology and hormonal milieu of male Sprague-Dawley rats’ reproductive organs,” Nigerian Journal of Health and Biomedical Sciences, vol. 2, no. 1, pp. 40–46, 2003. View at Google Scholar
  39. H. P. Misra and I. Fridovich, “The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase,” Journal of Biological Chemistry, vol. 247, no. 10, pp. 3170–3175, 1972. View at Google Scholar · View at Scopus
  40. H. Ohkawa, N. Ohishi, and K. Yagi, “Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction,” Analytical Biochemistry, vol. 95, no. 2, pp. 351–358, 1979. View at Google Scholar · View at Scopus
  41. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Assay of Protein, the original method,” Journal of Biological Chemistry, vol. 193, p. 265, 1951. View at Google Scholar
  42. H. Sies, “Strategies of antioxidant defense,” European Journal of Biochemistry, vol. 215, no. 2, pp. 213–219, 1993. View at Google Scholar · View at Scopus
  43. W. O. Nelson, “Mammalian spermatogenesis: effects of experimental cryptorchidism in the rat and non-descent of the testis in man,” Recent Progress in Hormone Research, vol. 6, pp. 29–62, 1951. View at Google Scholar
  44. T. Shikone, H. Billig, and A. J. W. Hsueh, “Experimentally induced cryptorchidism increases apoptosis in rat testis,” Biology of Reproduction, vol. 51, no. 5, pp. 865–872, 1994. View at Publisher · View at Google Scholar · View at Scopus
  45. K. Henriksen, H. Hakovirta, and M. Parvinen, “In-situ quantification of stage-specific apoptosis in the rat seminiferous epithelium: effects of short-term experimental cryptorchidism,” International Journal of Andrology, vol. 18, no. 5, pp. 256–262, 1995. View at Google Scholar · View at Scopus
  46. J. R. Davis and C. F. Firlit, “The germinal epithelium of cryptorchid testes experimentally induced in prepubertal and adult rats,” Fertility and Sterility, vol. 17, no. 2, pp. 187–200, 1966. View at Google Scholar · View at Scopus
  47. H. Kimura, Y. Yamada, Y. Morita, H. Ikeda, and T. Matsuo, “Dietary ascorbic acid depresses plasma and low density lipoprotein lipid peroxidation in genetically scorbutic rats,” Journal of Nutrition, vol. 122, no. 9, pp. 1904–1909, 1992. View at Google Scholar · View at Scopus
  48. R. Radi, J. S. Beckman, K. M. Bush, and B. A. Freeman, “Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide,” Archives of Biochemistry and Biophysics, vol. 288, no. 2, pp. 481–487, 1991. View at Publisher · View at Google Scholar · View at Scopus
  49. A. Rehman, C. S. Collis, M. Yang et al., “The effects of iron and vitamin C co-supplementation on oxidative damage to DNA in healthy volunteers,” Biochemical and Biophysical Research Communications, vol. 246, no. 1, pp. 293–298, 1998. View at Publisher · View at Google Scholar · View at Scopus
  50. T. Koizumi and Z. G. Li, “Role of oxidative stress in single-dose, cadmium-induced testicular cancer,” Journal of Toxicology and Environmental Health, vol. 37, no. 1, pp. 25–36, 1992. View at Google Scholar · View at Scopus
  51. Ö. Yaman, T. Soygür, E. Yilmaz, S. Elgün, A. Keskinege, and O. Göǧüş, “The significance of testicular reactive oxygen species on testicular histology in infertile patients,” International Urology and Nephrology, vol. 31, no. 3, pp. 395–399, 1999. View at Publisher · View at Google Scholar · View at Scopus
  52. M. Meydani, “Vitamin E,” The Lancet, vol. 345, no. 8943, pp. 170–174, 1995. View at Google Scholar · View at Scopus
  53. S. Devaraj, D. Li, and I. Jialal, “The effects of alpha tocopherol supplementation on monocyte function: decreased lipid oxidation, interleukin 1β secretion, and monocyte adhesion to endothelium,” Journal of Clinical Investigation, vol. 98, no. 3, pp. 756–763, 1996. View at Google Scholar · View at Scopus
  54. L. J. Van Tits, P. N. Demacker, J. De Graaf, H. L. Hak-Lemmers, and A. F. Stalenhoef, “α-Tocopherol supplementation decreases production of superoxide and cytokines by leukocytes ex vivo in both normolipidemic and hypertriglyceridemic individuals,” American Journal of Clinical Nutrition, vol. 71, no. 2, pp. 458–464, 2000. View at Google Scholar · View at Scopus
  55. N. Ferré, J. Camps, A. Paul et al., “Effects of high-fat, low-cholesterol diets on hepatic peroxidation and antioxidants in apolipoprotein E-deficient mice,” Molecular and Cellular Biochemistry, vol. 218, no. 1-2, pp. 165–169, 2001. View at Publisher · View at Google Scholar · View at Scopus
  56. A. H. Goldfarb, M. K. McIntosh, B. T. Boyer, and J. Fatouros, “Vitamin E effects on indexes of lipid peroxidation in muscle from DHEA- treated and exercised rats,” Journal of Applied Physiology, vol. 76, no. 4, pp. 1630–1635, 1994. View at Google Scholar · View at Scopus
  57. E. A. Meagher, O. P. Barry, J. A. Lawson, J. Rokach, and G. A. FitzGerald, “Effects of vitamin E on lipid peroxidation in healthy persons,” Journal of the American Medical Association, vol. 285, no. 9, pp. 1178–1182, 2001. View at Google Scholar · View at Scopus
  58. M. Reilly, N. Delanty, J. A. Lawson, and G. A. FitzGerald, “Modulation of oxidative stress invivo in chronic cigarette smokers,” Circulation, vol. 94, no. 1, pp. 19–25, 1996. View at Google Scholar · View at Scopus
  59. B. E. Leibovitz, M. L. Hu, and A. L. Tappel, “Lipid peroxidation in rat tissue slices: effect of dietary vitamin E, corn oil-lard and menhaden oil,” Lipids, vol. 25, no. 3, pp. 125–129, 1990. View at Google Scholar · View at Scopus
  60. G. Palumbo, F. Avanzini, C. Alli et al., “Effects of vitamin E on clinic and ambulatory blood pressure in treated hypertensive patients,” American Journal of Hypertension, vol. 13, no. 5, pp. 564–567, 2000. View at Google Scholar · View at Scopus
  61. C. G. Moertel, T. R. Fleming, and E. T. Creagan, “High-dose vitamin C versus placebo in the treatment of patients with advanced cancer who have had no prior chemotherapy. A randomized double-blind comparison,” New England Journal of Medicine, vol. 312, no. 3, pp. 137–141, 1985. View at Google Scholar · View at Scopus
  62. G. R. Buettner and P. L. Moseley, “EPR spin trapping of free radicals produced by bleomycin and ascorbate,” Free Radical Research Communications, vol. 19, no. 1, pp. S89–S93, 1993. View at Google Scholar · View at Scopus
  63. I. M. Drake, N. P. Mapstone, C. J. Schorah et al., “Reactive oxygen species activity and lipid peroxidation in Helicobacter pylori associated gastritis: relation to gastric mucosal ascorbic acid concentrations and effect of H pylori eradication,” Gut, vol. 42, no. 6, pp. 768–771, 1998. View at Google Scholar · View at Scopus
  64. J. A. North, A. A. Spector, and G. R. Buettner, “Cell fatty acid composition affects free radical formation during lipid peroxidation,” American Journal of Physiology, vol. 267, no. 1, pp. C177–C188, 1994. View at Google Scholar · View at Scopus
  65. L. S. Alexander-North, J. A. North, K. P. Kiminyo, G. R. Buettner, and A. A. Spector, “Polyunsaturated fatty acids increase lipid radical formation induced by oxidant stress in endothelial cells,” Journal of Lipid Research, vol. 35, no. 10, pp. 1773–1785, 1994. View at Google Scholar · View at Scopus
  66. H. Kappus, “Lipid peroxidation: mechanisms, enzymology and biological relevance,” in Oxidative Stress, H. Sies, Ed., pp. 273–310, Academic Press, New York, NY, USA, 1985. View at Google Scholar
  67. J. Chaudière and R. Ferrari-Iliou, “Intracellular antioxidants: from chemical to biochemical mechanisms,” Food and Chemical Toxicology, vol. 37, no. 9-10, pp. 949–962, 1999. View at Publisher · View at Google Scholar · View at Scopus
  68. A. C. Carr, B. Z. Zhu, and B. Frei, “Potential antiatherogenic mechanisms of ascorbate (vitamin C) and α-tocopherol (vitamin E),” Circulation Research, vol. 87, no. 5, pp. 349–354, 2000. View at Google Scholar · View at Scopus
  69. N. S. Dhalla, R. M. Temsah, and T. Netticadan, “Role of oxidative stress in cardiovascular diseases,” Journal of Hypertension, vol. 18, no. 6, pp. 655–673, 2000. View at Google Scholar · View at Scopus
  70. B. Sokmen, H. Basaraner, and R. Yanardag, “Combined effects of treatment with vitamin C, vitamin E and selenium on the skin of diabetic rats,” Human & Experimental Toxicology. In press.
  71. A. Sureda, M. D. Ferrer, A. Mestre, J. A. Tur, and A. Pons, “Vitamins C and E diet supplementation prevents neutrophil protein oxidation without affecting the adaptative response to exercise,” International Journal of Sport Nutrition and Exercise Metabolism. In press.
  72. A. Ascherio, E. B. Rimm, M. A. Hernán et al., “Relation of consumption of vitamin E, vitamin C, and carotenoids to risk for stroke among men in the United States,” Annals of Internal Medicine, vol. 130, no. 12, pp. 963–970, 1999. View at Google Scholar · View at Scopus
  73. L. H. Kushi, A. R. Folsom, R. J. Prineas, P. J. Mink, Y. Wu, and R. M. Bostick, “Dietary antioxidant vitamins and death from coronary heart disease in postmenopausal women,” New England Journal of Medicine, vol. 334, no. 18, pp. 1156–1162, 1996. View at Publisher · View at Google Scholar · View at Scopus
  74. H. N. Hodis, W. J. Mack, L. LaBree et al., “Serial coronary angiographic evidence antioxidant vitamin intake reduces progression of coronary artery atherosclerosis,” Journal of the American Medical Association, vol. 273, no. 23, pp. 1849–1854, 1995. View at Publisher · View at Google Scholar · View at Scopus