Table of Contents
ISRN Analytical Chemistry
Volume 2012 (2012), Article ID 908795, 5 pages
http://dx.doi.org/10.5402/2012/908795
Research Article

Development and Validation of a HPLC-UV Method for Simultaneous Determination of Five Sulfonylurea Herbicide Residues in Groundnut Oil Followed by Matrix Solid-Phase Dispersion

Department of Analytical Chemistry, International Institute of Biotechnology and Toxicology (IIBAT), Padappai, Kancheepuram 601301, India

Received 27 March 2012; Accepted 20 May 2012

Academic Editors: T. Bolanca, N. Chaniotakis, G. Drochioiu, N. Egashira, and G. Kardas

Copyright © 2012 T. Nageswara Rao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple, sensitive, and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of sulfonylurea herbicide residues (nicosulfuron, pyrazosulfuron ethyl, metsulfuron methyl, chlorsulfuron and azimsulfuron) in groundnut oil. The evaluated parameters included the type and amount of sorbent (silica gel, C 18 and florisil) and the nature of eluent (ethyl acetate, dichloromethane, and acetonitrile). The best results were obtained using 1.0 g of groundnut oil sample, 1.0 g of C 18 as sorbent, and 20 mL of ethyl acetate-dichloromethane (1 : 1, (v/v)). The method was validated using groundnut oil samples spiked with sulfonylurea herbicides at different concentration levels (0.05 and 0.5  πœ‡ g/mL). Average recoveries (using each concentration six replicates) ranged 90–97%, with relative standard deviations less than 3%, the concentration of calibration solutions was in the range 0.01–2.0  πœ‡ g/mL and limits of detection (LOD) as well as limits of quantification (LOQ) were 0.01  πœ‡ g/mL and 0.05  πœ‡ g/mL, respectively.