Table of Contents
ISRN Spectroscopy
Volume 2012, Article ID 918093, 7 pages
http://dx.doi.org/10.5402/2012/918093
Research Article

A Novel Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Cytochrome C and Forecast of Human Diseases

1Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, China
2Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou 363000, China
3Department of Food Safety Verification, Fujian Provincial Bureau of Quality and Technical Supervision, Zhangzhou 363000, China
4Department of Biochemistry, Fujian Education College, Fuzhou 350001, China

Received 29 August 2012; Accepted 11 November 2012

Academic Editors: C. Alvarez-Lorenzo, D. V. Konarev, and A. R. Türker

Copyright © 2012 Zhi-Yong Zheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The reaction between fullerenol (F-ol) and cytochrome C (Cyt C) could be carried out to form a nonphosphorescence compound using tween-80 as photosensitizer which causes the sharp quenching of room temperature phosphorescence (RTP) of F-ol. Bearing this in mind, a novel solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace Cyt C has been proposed in this study. Under the optimum conditions, the linear range of this method is    , which is directly proportional to of Cyt C-F-ol–tween-80 system, and the detection limit (DL) is . It has been applied to the determination of Cyt C in human serum and forecast of diseases, and the result matches with the enzyme-linked fluorescence immunoassay (ELISA). Meanwhile, the reaction mechanism of SSRTP for the determination of Cyt C and the enhancing effect of tween-80 on RTP of F-ol were also discussed.