Figure 6: PINK1 kinase activity is required for the mitochondrial translocation of Parkin. Wild-type (WT) or kinase-dead (KD) PINK1 together with GFP-Parkin (green) was introduced in mouse embryonic fibroblasts derived from a PINK1-deficient mouse. GFP-Parkin was located on the mitochondria (red; Tom 20) in cells expressing PINK1 WT but not PINK1 KD after CCCP treatment. Scale bar = 10 μm. Original data were provided by Shiba-Fukushima et al.