Real-Time PCR-Coupled CE-SELEX for DNA Aptamer Selection

<table>Bulk affinity assay of BSA and DNA. This electropherogram is the bulk affinity assay in which a high concentration of BSA (100 <i>μ</i>M) and DNA (3 <i>μ</i>M) was run together. The <svg height="9.8625002" id="M3" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 7.875 9.8625002" width="7.875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.8625)">
<g transform="translate(72,-64.11)">
<text transform="matrix(1,0,0,-1,-71.95,66.45)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑦</tspan>
</text>
</g>
</g>
</svg>-axis represents the absorbance at 280 nm, and the <svg height="7.1624999" id="M4" style="vertical-align:-0.11285pt" version="1.1" viewbox="0 0 8.7250004 7.1624999" width="8.7250004" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.1625)">
<g transform="translate(72,-66.27)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑥</tspan>
</text>
</g>
</g>
</svg>-axis is the run time to the absorbance detector. The tall peak starting around 40 minutes is the free BSA, and the smaller peak starting around 77 minutes is the free DNA. The collection window for the BSA-DNA complexes is boxed in black.</table>

International Scholarly Research Notices

Real-Time PCR-Coupled CE-SELEX for DNA Aptamer Selection

Figure 2