Research Article

Real-Time PCR-Coupled CE-SELEX for DNA Aptamer Selection

Figure 3

CE fractions collected and analyzed. (a) The time points at which the seven fractions were collected during the first round of CE selection are represented by the bars in the graph. The 𝑥 -axis for the bar graph and the electropherogram is the run time to the absorbance detector in minutes. The 𝑦 -axis of the electropherogram represents the absorbance at 280 nm. The 𝑦 -axes for the bar graphs are the number of RT-PCR cycles it took to reach 50% of the maximum or the midpoint cycle. The earlier the fraction reached the midpoint, the more DNA the fraction contained. The electropherogram above the bar graph is shown as a guide since the actual amount of BSA used for round 1 of selection is very low and it cannot be detected by CE. A supposed complex was detected in the second fraction collected during round 1 of selection (white bar). The supposed complex in the 2nd fraction of round 1 was analyzed in two test rounds using 100 μM BSA (b) or no BSA (c). Seven fractions were collected at the same time points as the seven fractions shown in (a) and the corresponding RT-PCR bar graphs of the midpoints are shown, with the 2nd fraction collected shown in white.
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