Real-Time PCR-Coupled CE-SELEX for DNA Aptamer Selection
Figure 4
Confirmation of CE-SELEX aptamer selection by EMSA. (a) Shown is the initial EMSA done with the BSA aptamer, I1-5, selected through RT-PCR coupled CE-SELEX, along with a negative control of the same length (“C” in the figure) described in Section 2. At 500 μM BSA a specific complex, formed between the BSA and DNA. Also, there is a higher band corresponding to nonspecific interaction at high concentrations of BSA in both the negative control and the aptamer I1-5 sequences. (b) EMSA of I1-5 and negative control with the modified binding conditions. The binding pattern was the same with these two different binding conditions and for clarity only the BSA at 500 μM is shown.
