Research Article

Real-Time PCR-Coupled CE-SELEX for DNA Aptamer Selection

Table 2

Sequences of potential aptamers for BSA. The table contains the sequences of the potential aptamers from each of the pooled groups of clones that showed binding to BSA by RT-PCR analysis. These sequences were ordered as salt-free oligonucleotides and analyzed using electrophoretic mobility shift assays (EMSAs), and sequences that showed binding are bolded. I1-5, boxed in black, was chosen for further analysis.

Potential aptamerInternal sequence

O3-9CAGTCACCTCAGCGTCCACAATTATGGCAGCGCGCG
G2-1TTTATTGGGGGAATATAACCGATCATGTGGCGGTTA
G2-2ATACAATGATTTGCACACCGGGTGGCGTGAATTGT
G2-3GGATTGTGGTAAGCTCTATCTACCTTGCTACGCCC
G2-4GGCTGATCGACAATAAGCGTGCCGGCCCTCAACTCC
G2-5TCCGATGCCACACTTGACGTGCTGGGTTGGCTGCGT
G2-6AGCGAGAGGCGACTTCATTATTGGGAGGTGGCTTCG
G2-8AGTAGGTGTTATGAGCACGACCGGTTTACTTCACG
G2-9GGAGTGACTGGAGGATCTGGTCCCCGGAGTTCCTTG
G2-11GCATGTGGTGCTTCTTGACCGCCTGCGCCTGCGGGT
R2-6AACGACTGGACTCCGTCATACAGTTTGGGGAAAGG
R2-8TCCCTACCCGGTGTTTGAAACGGCCGTAAGGAACTT
R2-10GATAGTAAGACCTGATTGGGGTCAAGCTAACGTCGA
R2-11GCTCGCGTTGTAAGCATTTGTTGGNCGAGATAGGCG

I1-5GCCCGCCGTGGCTGGGTCTTCCTTGGTCGGTCTAC

I1-11GTATCTACAGGGTGGCCAGGGCGCAACCGGGCGAGT
I1-12TGGCTAGCGATGTATTCCGTTTTGGGGATAAATCCA