Table 3: Danshen effects on the numbers of peripheral blood leukocyte subpopulations before and after LM infection.

Danshen
(% of daily diet)
Neutrophils (cells/ L)Monocytes (cells/ L)Lymphocytes (cells/ L)NK cells (cells/ L)
PreinfectionPostinfectionP  value
PreinfectionPostinfectionP  valuePreinfectionPostinfectionP  valuePreinfectionPostinfectionP  value

02747 ± 2792038 ± 908ns717 ± 146885 ± 371ns4865 ± 6941258 ± 5230.018193 ± 32214 ± 57ns
0.51903 ± 1472928 ± 1462ns798 ± 951213 ± 425ns4509 ± 5941841 ± 5080.014136 ± 26653 ± 1180.029
13022 ± 8902128 ± 399ns912 ± 1341051 ± 134ns4848 ± 7371242 ± 2460.004219 ± 82543 ± 191ns
22749 ± 13442414 ± 247ns484 ± 711220 ± 2530.0123419 ± 5181890 ± 460ns104 ± 22321 ± 700.006

After ingesting 0–2% Danshen for 70 days, peripheral blood was collected before and after LM infection. The sample was prepared in a TruCOUNT tube and analyzed by flow cytometry. The absolute number of neutrophils, monocytes, lymphocytes, and NK cells was calculated based on four to nine mice from 2–4 separated experiments. Data are presented as mean ± SEM.