Innate Immunity in Lobsters: Partial Purification and Characterization of a Panulirus cygnus Anti-A Lectin

Table 1

Haemagglutination of normal and papain-treated erythrocytes by P. cygnus haemolymph and affinity chromatography purified lectin.

	HA titre^a
	Untreated erythrocytes		Papain-treated erythrocytes
Type of erythrocyte	Haemolymph	Purified lectin^b	Haemolymph	Purified lectin^b
Human 0	16	20	16	20
Human A	32	40	64	80
Human B	16	20	16	20
Chicken	0	0	16	20
Rat	4,096	5,120	128,000	128,000
Sheep	16	20	64	80

^aTitres were determined as described in the text.
^bLectin was purified by affinity chromatography on REM-Sepharose, with sucrose-urea as eluant. The eluate was diluted 1 : 10 and then titrated. The A₂₈₀ of the undiluted lectin preparation was 0.2.