Isoflurane Enhances the Moonlighting Activity of GAPDH: Implications for GABAA Receptor Trafficking
Figure 2
Isoflurane enhances GAPDH-induced ULV aggregation/fusion. (a) GAPDH was first exposed to isoflurane (0.1 mM) prior to incubation with ULVs and subsequent measurement of right-angle light scattering (RALS). Conditions are given on the -axis. Data are presented visually to indicate size, which is given on the -axis. The vesicles sizes are scaled relative to the plus isoflurane control. Data are presented as means of triplicate measurements from a representative experiment. Error bars are omitted for clarification, and the within-group significance is shown to the right of the shapes. (b) GAPDH was first exposed to isoflurane (0.1 mM) prior to incubation with ULVs (phosphatidylserine-rich) and subsequent analysis by light microscopy. Panels: (1) ULVs only; (2) ULVs with GAPDH minus isoflurane; (3) ULVs without GAPDH plus isoflurane; (4) ULVs with GAPDH plus isoflurane. Bar, 5 μm. Magnification, 400X.
