Glycosaminoglycans Modify Elastase Action In Vitro and Enhance Elastase-Induced Cell Death in Cultured Fibroblasts
Figure 6
Effect of GAGs on DNA fragmentation of NCM-exposed fibroblasts. Cells were incubated with NCM (HNE activity of 8.0 or 15 nM), in the absence or presence of C6S or HS (10 μM), for 16 h in the cell culture conditions. After this period, cells were collected by trypsinization, stained by PI, in the presence of Nonidet P-40, and analyzed by flow cytometry. A total of 10,000 events were registered. (a) statistically differs from Control (untreated cells); (b) differs from HS group; (c) differs from C6S group; (d) differs from HNE group ().
