Hemostatic and Wound Healing Properties of Chromolaena odorata Leaf Extract
Figure 2
Expression of genes involved in wound healing activity. (a) Balb/c 3T3 fibroblasts were grown in the presence of indicated SWE for 6 h and 12 h. The cell lysates were electrophoresed in SDS-PAGE, and several genes and proteins were examined. Actin was used as a control. (b) Scu and stigmasterol were added to Balb/c 3T3 cells for 12 h. Western blot was carried out as described in the Materials and Methods section. (c) Balb/c 3T3 cells were treated with SWE for 12 h and HO-1 mRNA expression was measured. Reverse-transcriptase PCR was performed, and the data represent three independent experiments. GAPDH was used as control.
