Research Article

Improvement of Polyunsaturated Fatty Acid Production in Echium acanthocarpum Transformed Hairy Root Cultures by Application of Different Abiotic Stress Conditions

Table 2

Total lipid (TL) amounts (mg/g DW) and lipid classes profile (%) of Echium acanthocarpum hairy roots harvested at sampling points 4 and 5 growing under abiotic stress (low temperature in cultures C1–C4, osmotic stress in cultures C2 and C4, and glucose as carbon source in cultures C3 and C4).
(a) Data of sampling point 4

Sampling point 4Culture B1Culture C1Culture C2Culture C3Culture C4

TL (mg/g DW)
PC
PS + PI
PG
PE
Polar lipid
Neutral lipid
unknown

(b) Data of sampling point 5

Sampling point 5Culture B1Culture C1Culture C2Culture C3Culture C4

TL (mg/g DW)
PC
PS + PI
PG
PE
Polar lipid
Neutral lipid
unknown

Significant differences ( ) between cultures given by Student’s t-test when comparing the mean of percentages of the lipid classes for cultures B1 and C1.
Significant differences of TL values, also showing homogeneous subclusters as indicated by Tuckey's test when comparing the mean of TL values.
TL: total lipid; PC: phosphatidylcholine; PS + PI: sum of phosphatidylserine and phosphatidylinositol; PG: phosphatidylglycerol; PE: phosphatidylethanolamine.
Percentage values were transformed by the arcsin transformation prior to statistical analysis.
Values represent the mean of three independent experiments ( ) ± SD.
Entry of culture B1 for sampling point 5 is empty because data were collected only for sampling 4.