International Scholarly Research Notices / 2013 / Article / Tab 5

Research Article

Improvement of Polyunsaturated Fatty Acid Production in Echium acanthocarpum Transformed Hairy Root Cultures by Application of Different Abiotic Stress Conditions

Table 5

Total lipid content (TL; mg/g DW), total fatty acid content (FA, mg/g DW), and the percentage of each fatty acid (%) of Echium acanthocarpum hairy roots at sampling point 4 cultured for 20 days (culture B1) or 45 days (cultures C1–C4).

Sampling point 4
B1C1C2C3C4

TL (mg/g DW)
FA (mg/g DW)
Fatty acids
 14:0 ND
 16:0
 18:0
 18:1n-9
 18:1n-7
 18:2n-6 (LA)
 18:3n-6 (GLA)
 18:3n-3 (ALA)
 18:4n-3 (SDA)
 20:0
 22:0
 24:0
 Unknown

GLA + SDA
Total saturated FA
Total monoene FA
n-9
n-6
n-3
n-3/n-6
6-des index (n-6)
6-des index (n-3)
DBI

ND: Not detected. n-6 and n-3 6-desaturation indexes were calculated as 18:3n-6/(18:3n-6 + 18:2n-6) and 18:4n-3/(18:4n-3 + 18:3n-3), respectively.
Double bond index (DBI) was calculated as [(%18:1n) + 2   (%18:2n) + 3   (%18:3n) + 4   (18:4n)]/100. Values represent the mean of three replicates ( ) SD.