Table of Contents
ISRN Neuroscience
Volume 2013, Article ID 170316, 4 pages
Research Article

Traveled Distance Is a Sensitive and Accurate Marker of Motor Dysfunction in a Mouse Model of Multiple Sclerosis

1Medical Research Institute, Tokyo Women’s Medical University, Tokyo 162-8666, Japan
2Kita Medical and Rehabilitation Center for Disabled, Tokyo 114-0033, Japan

Received 27 September 2013; Accepted 14 November 2013

Academic Editors: C. Comi, E. M. Mowry, and M. Sasaki

Copyright © 2013 Takako Takemiya and Chisen Takeuchi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Multiple sclerosis (MS) is a common central nervous system disease associated with progressive physical impairment. To study the mechanisms of the disease, we used experimental autoimmune encephalomyelitis (EAE), an animal model of MS. EAE is induced by myelin oligodendrocyte peptide, and the severity of paralysis in the disease is generally measured using the EAE score. Here, we compared EAE scores and traveled distance using the open-field test for an assessment of EAE progression. EAE scores were obtained with a 6-step observational scoring system for paralysis, and the traveled distance was obtained by automatic trajectory analysis of natural exploratory behaviors detected by a computer. The traveled distance of the EAE mice started to decrease significantly at day 7 of the EAE process, when the EAE score still did not reflect a change. Moreover, in the relationship between the traveled distance and paralysis as measured by the EAE score after day 14, there was a high coefficient of determination between the distance and the score. The results suggest that traveled distance is a sensitive marker of motor dysfunction in the early phases of EAE progression and that it reflects the degree of motor dysfunction after the onset of paralysis in EAE.