Table of Contents
ISRN Chromatography
Volume 2013, Article ID 183654, 5 pages
Research Article

Estimation of Abamectin Residues Present in Tea: High-Performance Liquid Chromatography Technique

UPASI Tea Research Foundation, Tea Research Institute, Coimbatore District, Valparai, 642127 Tamil Nadu, India

Received 19 February 2013; Accepted 19 March 2013

Academic Editors: J.-F. Jen, A. I. Suarez, and A. Vazquez

Copyright © 2013 Madasamy Kottiappan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple, reliable, and sensitive method was based on high-performance liquid chromatography (HPLC) was developed and validated for the estimation of abamectin residues present in tea. The abamectin residues extracted with acetone-water mixture (70 : 30, v/v) and derivatised with 1-methylimidazole (1-MIM) and trifluoroacetic anhydride (TFAA) were estimated by HPLC using fluorescence detector (FLD). The technique was validated in terms of linearity, precision, recovery, specificity, limit of detection (LOD), and limit of quantification (LOQ). A good linear relationship ( ) was absorbed in the abamectin concentration range from 0.01 to 1.0 μg mL−1. The limit of detection and limit of quantification of the method were 0.01 and 0.02 μg g−1, respectively. The average recoveries of the pesticide from black tea and dried green leaves ranged from 83.3 to 103.8% and 83.8 to 98.0%, respectively.