Effect of pulse treatment with Cd in CHOC VDCC-expressing and wild-type CHO cells. Microphotographs of control (a, b) and Cd-treated (c, d) cells: (a, c) VDCC-expressing CHOC cells and (b, d) wild-type CHO cells. Both cell types were incubated in 100 μM Cd for 60 minutes in the presence of 30 mM KCl. Pictures were taken 24 h after wash of the metal. . The graph (e) shows the effect of a 30 min pulse treatment with Cd on cell adhesion, as a function of concentration. Trypan-blue excluding adherent cells were counted 24 h after wash of the metal. Points are average of 3 experiments in the same condition in CHOC (filled circles) and wild-type CHO (empty circles) and were best fitted to the function. , where is the number of adherent cells after Cd treatment normalized to the number of adherent cells in control culture; [Cd] is the concentration of Cd and ED50 is the concentration of Cd that causes detachment from the substrate of 50% of cells. The best fit yielded for CHOC and 43 μM for wild-type CHO. The two curves are overlapped. In contrast with the different appearance, the two cell types were similarly affected by Cd treatment.
