Total Cd accumulation measured by FAAS in CHOC and wild-type CHO cells. Both cell types were incubated for 1 hour in medium containing 0, 50, or 100 Cd and other modifiers. Cd determination was normalized to the cell volume (see text). Treatments were as follows: control 0 Cd (basal), 60 min Cd (Cd), (K), , and 1 BayK (B), and 1 nimodipine (N). Depolarization (treatment K) did not increase significantly the intracellular concentration of Cd (). In CHOC cells, but not in wild-type CHO, BayK significantly enhanced and nimodipine significantly reduced Cd accumulation, both with (*), with respect to the same treatments in depolarization. Bars represent mean in at least 10 experiments in each group.
