Sequentially Integrated Optimization of the Conditions to Obtain a High-Protein and Low-Antinutritional Factors Protein Isolate from Edible Jatropha curcas Seed Cake

Table 3

Box-Behnken response surface design and response results.

Extraction temperature (°C)	[NaCl]; (mol L^− 1)	Precipitation pH	Protein; (g 100 g^−1 isolate)	Total phenolics; (mg 100 g^−1 sample)	Phytate; (mg 100 g^−1 sample)	Saponins; (mg 100 g^−1 sample)
40	4.5	0.3	87.13	546	5218	248
40	4.5	0.3	87.07	500	5229	244
40	4.0	0.0	89.89	685	6737	293
20	4.5	0.6	87.75	405	3346	239
60	4.5	0.6	87.58	452	4019	284
60	4.0	0.3	93.96	636	3395	303
40	5.0	0.0	89.62	566	5458	196
40	5.0	0.6	87.69	319	4731	250
20	4.5	0.0	87.86	694	5025	231
40	4.5	0.3	87.04	571	5308	245
60	5.0	0.3	93.41	379	2821	230
20	4.0	0.3	94.22	435	4626	170
40	4.0	0.6	91.73	467	3878	213
40	4.5	0.3	87.15	547	5431	243
20	5.0	0.3	96.69	379	2963	172
60	4.5	0.0	93.94	517	6170	221
40	4.5	0.3	87.18	441	4933	216