Table of Contents
ISRN Virology
Volume 2013, Article ID 207580, 5 pages
Research Article

A Comprehensive Study on the 2012 Dengue Fever Outbreak in Kolkata, India

1Calcutta School of Tropical Medicine, 108 C. R. Avenue, Kolkata 700073, India
2Maulana Azad Medical College, Bahadur Shah Zafar Marg, New Delhi, Delhi 110002, India
3NRHM, Government of West Bengal, Swasthya Bhavan Sector V, Salt Lake, Kolkata 700091, India

Received 13 June 2013; Accepted 17 July 2013

Academic Editors: A. Mastino and J. Stephenson

Copyright © 2013 Bhaswati Bandyopadhyay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Dengue viruses (DV) belong to the family Flaviviridae, with four serotypes referred to as DV-1, DV-2, DV-3, and DV-4. A large-scale outbreak of dengue fever occurred in 2012 involving several districts of West Bengal. Objective. To present a comprehensive picture of the dengue fever outbreak in 2012 and to identify the prevailing serotypes. Materials and Methods. Serum samples were collected from suspected dengue fever cases. Samples from fever cases <5 days duration were tested for dengue NS1 antigen employing Pan Bio (Australia) NS1 ELISA kit. Serum samples of ≥5 days fever were tested for dengue-specific IgM by MAC ELISA test kit prepared by the National Institute of Virology Pune, India. Serotyping of dengue samples was done by dengue-specific reverse transcriptase polymerase chain reaction (RT-PCR). Results. The number of dengue cases in 2012 clearly outnumbered the dengue cases in 2010 and 2011. The majority of the cases were in the age group 11–30 years with a male preponderance. Outbreak occurred during the months of Aug.–Nov. indicating increased vector transmission in the monsoon and postmonsoon periods. The prevailing serotypes in this outbreak were Den1, Den3, and Den4.