A Recent Review on Chemiluminescence Reaction, Principle and Application on Pharmaceutical Analysis

Figure 5

Basic steps in a CL process: (a) the sample and reagent(s) are introduced is the reaction cell and the final reagent is injected to initiate the CL emission, then lights is monitored by the detector, (b) curve showing CL intensity as a function of time after reagents mixing to initiate the reaction (the decay of the signal is due to the consumption of reagents and changes in the CL quantum efficiency with time); (c) a calibration function is established in relation to increasing analyte concentrations.