Table of Contents
ISRN Toxicology
Volume 2013, Article ID 242345, 6 pages
Research Article

Inhibition of Nitric Oxide Synthase by L-NAME Promotes Cisplatin-Induced Nephrotoxicity in Male Rats

1Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan 81745, Iran
2Department of Physiology, Isfahan University of Medical Sciences, Isfahan 81745, Iran
3Isfahan-MN Institute of Basic & Applied Sciences Research, Isfahan 81546, Iran
4Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan 81745, Iran
5Department of Internal Medicine, Isfahan University of Medical Sciences, Isfahan 81745, Iran

Received 26 June 2013; Accepted 28 July 2013

Academic Editors: J. P. Petzer, M. M. Sayed-Ahmed, and D. Wu

Copyright © 2013 Fatemeh Moslemi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. Nitric oxide (NO) has numerous important functions in the kidney. The role of NO in cisplatin (CP)-induced nephrotoxicity is not completely understood. This study was designed to determine the role of NO synthase inhibitor (L-NAME) on the severity of CP-induced nephrotoxicity in rats. Methods. Sixty four male (M) and female (F) Wistar rats were randomly divided into eight groups. The sham groups (group 1, male, and group 2, female, ) received saline. Groups 3 (male, ) and 4 (female, ) were treated with L-NAME (4 mg/kg, i.p.), and groups 5 (male, ) and 6 (female, ) received CP (3 mg/kg) for 7 days. Groups 7 (male, ) and 8 (female, ) were treated with L-NAME and CP for 7 days. Results. The CP-alone treated rats showed weight loss and increase in serum levels of blood urea nitrogen (BUN) and creatinine (Cr). Coadministration of L-NAME and CP did not improve weight loss, and it increased the levels of BUN and Cr in male but not in female rats ( ). CP alone increased kidney damage significantly ( ), however, the damage induced by combination of CP and L-NAME was gender-related. Conclusion. NOS inhibition by L-NAME increased CP-induced nephrotoxicity, which was gender-related.