Biofilm-Mediated Enhanced Crude Oil Degradation by Newly Isolated Pseudomonas Species

Table 2

Preliminary biochemical tests for isolated bacterial strains.

Characteristics	KPW.1-S1	HRW.1-S3	DSW.1-S4
Gram nature	Negative	Negative	Negative
Shape	Rod	Coccobacillary	Coccobacillary
Diffusible pigments	+	+	−
Motility	+	+	+
Citrate utilization	++	++	++
Lysine utilization	+	+	+
Ornithine utilization	+	+	+
Urease test	−	−	−
Phenylalanine test	NC	NC	NC
Nitrate reduction	NC	NC	NC
H2S production	+++	++	+
Production of acid by; utilization of sugar*
(i) Glucose	−	−	−
(ii) Adonitol	−	−	−
(iii) Lactose	−	−	−
(iv) Arabinose	−	−	−
(v) Sorbitol	−	−	−

NC: not conclusive.
*The test attributed the change of the color of pH indicator by production of acid and gas when grown in presence of various sugars.