Monocyte Migration Driven by Galectin-3 Occurs through Distinct Mechanisms Involving Selective Interactions with the Extracellular Matrix
Figure 1
Galectin-3 binds to the surface of human monocytes, an event inhibited by the specific sugar. Human monocytes ( cells) were incubated for 1 h at 4°C with biotin-conjugated hrGal-3 (25 g/mL), in the presence of 10 mM lactose or sucrose. (a) By flow cytometry, CD14+ gated cells were analyzed for hrGal-3-biotin staining after reaction with streptavidin-FITC. Histograms represent the number of hrGal-3 positive cells. (b) Monocytes incubated with hrGal-3-biotin were analyzed by confocal microscopy, after incubation with anti-CD14-FITC antibody and streptavidin-Alexa Fluor 594. Results are representative from three independent assays.
