Identification of PDZ Domain Containing Proteins Interacting with 1.2 and PMCA4b
Figure 2
PDZ Domain Array III. (a) Schematic representation of the TranSignal PDZ Domain Array III. 100 ng of the PDZ domain containing proteins are spotted in duplicate on the array. Pos: positive control (Histidine-tagged ligand), negative control-Glutathione-S-Transferase (GST). (b) PDZ Domain Array III was incubated with the bacterial lysate containing the Histidine-tagged recombinant protein, pEXP-Cav1.2. (c) PDZ Domain Array III was incubated with the bacterial lysate containing the Histidine-tagged recombinant protein, pEXP-PMCA4b. Both bacterial extracts had a concentration of 5 μg/mL. PDZ domain and ligand interactions were visualized with anti-Histidine antibodies.
