284830.fig.005a
(a)
284830.fig.005b
(b)
Figure 5: (a) Activity gel of superoxide dismutase (SOD) in native PAGE on 10% acrylamide gels of leaf extracts of six grass pea (Lathyrus sativus L.) genotypes at 60 days after commencement of 150 mM NaCl-treatment; Lane 1—control plants (Cu/Zn SOD I and II), lane 2—variety B1 (Cu/Zn SOD I and II), lane 3—variety BioL-212 (Cu/Zn SOD I and II), lane 4—PUSA-90-2 (Mn SOD I and II, Cu/Zn SOD I and II), lane 5—WBK-CB-14 (Mn SOD I and II, Cu/Zn SOD I and II), lane 6—LR3 mutant (Mn SOD I, Cu/Zn SOD I and II), and lane 7—LR4 mutant (Fe SOD, Cu/Zn SOD I and II), and (b) inhibitor studies with H2O2 and KCN and visualization of SOD isoforms in native PAGE of leaf extracts of grass pea (Lathyrus sativus L.) variety PUSA-90-2 (lanes 1 and 2), WBK-CB-14 (lanes 3 and 4), LR3 mutant (lanes 5 and 6), and LR 4 mutant (lanes 7 and 8) at 60 days after commencement of salt treatment. The control plants, variety B1 and BioL-212 containing only Cu/Zn SOD I and II showed no bands in inhibitor study and thus not presented.