Table of Contents
ISRN Entomology
Volume 2013 (2013), Article ID 286182, 5 pages
http://dx.doi.org/10.1155/2013/286182
Research Article

Vouchering of Forensically Important Fly Specimens by Nondestructive DNA Extraction

1Division of Medical Entomology, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Osong 363-700, Republic of Korea
2Department of Legal Medicine, College of Medicine, Korea University, 126-1 Anam-dong 5-ga, Seongbuk-gu, Seoul 136-705, Republic of Korea
3Beijing Scales Forensic Center, 100 Chaoyangru, Chaoyang-qu, Beijing 100025, China

Received 12 November 2012; Accepted 28 November 2012

Academic Editors: J. D. Andrade Filho, A. I. Kehr, and J. Klimaszewski

Copyright © 2013 Seong Yoon Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

DNA extraction frequently requires destruction of whole samples. However, when the sample is very rare or has taxonomic importance, nondestructive DNA extraction is required for preservation of voucher specimens. In the case of arthropod specimens, minor anatomical structures such as a single leg or a single wing are often sacrificed instead of the whole body for DNA extraction. In an attempt to save the entire anatomical structure of specimens, several authors tried to brew the whole specimen in a lysis buffer and to extract DNA from the “soup.” We applied this nondestructive DNA extraction technique to a forensically important blowfly species, Phaenicia sericata. With nondestructive DNA extraction, a satisfactory quantity and quality of DNA for PCR amplification was obtained with only minimal anatomical disruptions that do not alter the morphologic identification. This nondestructive method may be applicable to DNA extraction of rare samples as well as vouchering of regular fly samples.