Table of Contents
ISRN Toxicology
Volume 2013 (2013), Article ID 316075, 8 pages
http://dx.doi.org/10.1155/2013/316075
Research Article

Nanosized Zinc Oxide Induces Toxicity in Human Lung Cells

1Pharmacology and Toxicology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474001, India
2Saveetha University, Chennai 600077, India
3Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Mysore 570011, India

Received 27 May 2013; Accepted 14 July 2013

Academic Editors: G. Ramesh and G. S. Shukla

Copyright © 2013 Devashri Sahu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Zinc oxide nanoparticles (ZnO-NPs) are increasingly used in sunscreens, biosensors, food additives, pigments, rubber manufacture, and electronic materials. With the wide application of ZnO-NPs, concern has been raised about its unintentional health and environmental impacts. This study investigates the toxic effects of ZnO-NPs in human lung cells. In order to assess toxicity, human lung epithelial cells (L-132) were exposed to dispersion of 50 nm ZnO-NPs at concentrations of 5, 25, 50, and 100 μg/mL for 24 h. The toxicity was evaluated by observing changes in cell morphology, cell viability, oxidative stress parameters, DNA damage analysis, and gene expression. Exposure to 50 nm ZnO-NPs at concentrations between 5 and 100 μg/mL decreased cell viability in a concentration-dependent manner. Morphological examination revealed cell shrinkage, nuclear condensation, and formation of apoptotic bodies. The oxidative stress parameters revealed significant depletion of GSH level and increase in ROS levels suggesting generation of oxidative stress. ZnO-NPs exposure caused DNA fragmentation demonstrating apoptotic type of cell death. ZnO-NPs increased the expression of metallothionein gene, which is considered as a biomarker in metal-induced toxicity. To summarize, ZnO-NPs cause toxicity in human lung cells possibly through oxidative stress-induced apoptosis.