Table of Contents
ISRN Chromatography
Volume 2013, Article ID 328157, 8 pages
Research Article

Cefpodoxime Proxetil: A New Stability Indicating RP-HPLC Method

1Gokaraju Rangaraju College of Pharmacy, Osmania University, Bachupally, Hyderabad 500 090, India
2Jawaharlal Nehru Technological University, Hyderabad 500 085, India

Received 24 April 2013; Accepted 29 May 2013

Academic Editors: J. A. P. Coelho, B. Fernandez De Simon, and M. Hassan

Copyright © 2013 Ceema Mathew et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present work describes the development of a sensitive and economic stability indicating high performance liquid chromatographic (HPLC) method for the determination of cefpodoxime proxetil (CP) as bulk drug and as pharmaceutical formulation. Both R and S isomers of the drug were separated using Phenomenex (  mm, 5 μm particle size) ODS column with a flow rate of 1 mL min−1 and an SPD 20 A UV detector to monitor the eluate at 252 nm. The isocratic method used a mobile phase consisting of methanol and phosphate buffer of pH 4.0 in the ratio 65 : 35. The linear regression analysis data for the calibration plots showed good linear relationship with in the working concentration range of 5–100 μg mL−1. The LOD and LOQ were 53 and 160 ng mL−1, respectively. CP was subjected to stress degradation using acid, alkali, hydrogen peroxide, dry heat, wet heat, and UV light. The standard drug peaks were well resolved from the degradation products’ peaks with significantly different retention time (Rt), and the resolution factor for the R and S isomers of CP was found to be greater than 2.