High-Performance Liquid Chromatographic Method for Analysis of Emtricitabine in Rat Plasma: Method Development, Validation and Application to a Pharmacokinetic Study

Table 2

Stability of emtricitabine in rat plasma (𝑛=6).

Sample condition	Spiked concentration (μg/mL)	Mean determined concentration; (µg/mL)	Accuracy (%)
Bench-top stability^♣	0.1	0.094	94.00
	1.0	0.98	98.00
	2.5	2.42	96.80
Freeze-thaw stability^▸	0.1	0.099	99.00
	1.0	1.03	103.00
	2.5	2.46	98.40
One-week stability^♦	0.1	0.092	92.00
	1.0	0.89	89.00
	2.5	2.38	95.20

♣Exposed at ambient temperature (25°C) for 4 h.
^▸After three freeze-thaw cycles.
^♦Stored at −16°C.