Stability-Indicating Validated Novel RP-HPLC Method for Simultaneous Estimation of Methylparaben, Ketoconazole, and Mometasone Furoate in Topical Pharmaceutical Dosage Formulation

Table 3

Method precision, intermediate precision result, LOD, LOQ evaluation, and linearity data for MP, KT, and MF.

Parameter	MP	KT	MF
Precision day-1 (𝑛=6); (% Assay ± SD; % RSD)	100.5 ± 0.48; 0.48	99.7 ± 0.43; 0.43	100.2 ± 0.76; 0.75
Intermediate precision day-2 (𝑛=6); (% Assay ± SD; % RSD)	100.0 ± 0.32; 0.32	100.4 ± 0.17; 0.17	101.3 ± 0.59; 0.58
LOD (𝜇g/mL)	0.033	0.204	0.123
LOQ (𝜇g/mL)	0.12	0.67	0.42
Linearity range (𝜇g/mL)	0.12–15.2	0.67–149.4	0.42–7.6
Correlation coefficient	0.9999	0.9999	0.9999
Intercept (𝑎)	1485.841	415.816	−1614.022
Slope (𝑏)	72985.066	17217.396	35584.579
Bias at 100% response	−0.202	0.024	−0.914