Flow Cytometric Measurement of Aneuploid DNA Content Correlates with High S-Phase Fraction and Poor Prognosis in Patients with Non-Small-Cell Lung Cancer

<table>DNA content (ploidy) analysis in non-small-cell lung carcinoma samples measured by the flow cytometric profile and analyzed by ModFit LT software. Cells prepared by the Hedley method [<a href="/journals/isrn/2013/354123/#B16">16</a>] and stained with propidium iodide. (a) DNA histogram of diploid tumor. Note that a single G<sub>0</sub>/G<sub>1</sub> population. (b) DNA histogram of aneuploid tumor. Observe the appearance of one separate second G<sub>0</sub>/G<sub>1</sub> population to the right of the first G<sub>0</sub>/G<sub>1</sub> peak. The percentage of cells in S-phase of cell cycle was calculated from each DNA histogram. In case of aneuploid histograms, percentage of cells in S-phase was only shown for aneuploid population. Note that DNA aneuploid tumors have higher SPF than DNA diploid tumors do (<svg height="11.375" id="M21" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 75.025002 11.375" width="75.025002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>; Fisher's exact test).</table>

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Figure 2

Figure 2: Flow Cytometric Measurement of Aneuploid DNA Content Correlates with High S-Phase Fraction and Poor Prognosis in Patients with Non-Small-Cell Lung Cancer 

Figure 2 | Flow Cytometric Measurement of Aneuploid DNA Content Correlates with High S-Phase Fraction and Poor Prognosis in Patients with Non-Small-Cell Lung Cancer 