Table of Contents
ISRN Pharmacology
Volume 2013, Article ID 462918, 7 pages
http://dx.doi.org/10.1155/2013/462918
Research Article

Improved HPLC Method for the Determination of Moxifloxacin in Application to a Pharmacokinetics Study in Patients with Infectious Diseases

1Pharmacy Department, The Third Central Hospital, No. 83 Jintang Road, Hedong District, Tianjin 300170, China
2Basic Medical Department, Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin 300070, China
3Pharmacy Department, First Center Hospital, No. 24 Fukang Road, Nankai District, Tianjin 300192, China
4Infectious Disease Department, First Center Hospital, Tianjin, China
5School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, China

Received 8 April 2013; Accepted 28 April 2013

Academic Editors: M. Alkondon, G. Froldi, and K. Wada

Copyright © 2013 Nan Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To develop a simple and rapid high-performance liquid chromatography (HPLC) method for measuring moxifloxacin concentration in human plasma. Methods. Following a single step liquid-liquid extraction, analytes along with an internal standard (IS) were separated using an isocratic mobile phase of 0.1% triethylamine (adjusted pH to 4.8 with phosphoric acid)/acetonitrile (80/20, v/v) at flow rate of 1 mL/min on reverse phase Kromasil C18 column (250 mm × 4.6 mm, 5 μm) at room temperature. Results. Total analytical run time for selecting moxifloxacin was 15 min. The assays exhibited good linearity ( ) over the studied range of 25 to 5000 ng/mL. The absolute recovery rate of low, medium, and high concentrations were 69.88%, 78.86%, and 78.51%, respectively. The relative recovery rates were 98.50%, 96.61%, and 101.79%, respectively. Coefficient of variation and error at both of the intraday and interday assessments were less than 4.7%. Conclusions. The results indicated that this method is a simple, rapid, precise and accurate assay for the determination of moxifloxacin concentrations in human plasma. This validated method is sensitive and reproducible enough to be used in pharmacokinetic studies.