PKC-Dependent Signaling Pathways within PAG and Thalamus Contribute to the Nitric Oxide-Induced Nociceptive Behavior

Figure 4

NO donors modulated CREB and STAT1 expression and phosphorylation. GNT and SNP modulated cerebral CREB protein levels as indicated by the CREB downregulation within PAG lysate (a) and thalamus (b). An upregulation of phosphorylated CREB (pCREB) following NO donors’ administration was observed in the PAG (c) and thalamus (d). NO donors induced upregulation of phosphorylated STAT1 (pSTAT1). pSTAT1 protein levels were increased 1, 2 and 4 h after SNP treatment within PAG (e) and thalamus (f). ^∗𝑃<0.05,^∗∗𝑃<0.01,^∗∗∗𝑃<0.001 compared with vehicle-treated control group (c).

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